Up-regulation of the G3PDH ʻhousekeeping’ gene by estrogen

Abstract

Proteomic and genomic studies commonly involve the assessment of mrna levels using reverse transcription- polymerase chain reaction (Pcr) and real-time quantitative Pcr. an internal standard rna is fundamentally analyzed along with the investigated mRNA to document the specificity of the effect(s) on mrna and to correct for inter-sample variations. in our studies implementing estrogen treatments on different cell lines, we initially used glyceraldehyde-3- phosphate dehydrogenase (G3PdH) as an internal standard. However, the results of PCR amplification demonstrated that 17β-estradiol enhanced the expression of the G3PdH gene, rendering it impossible to use G3PdH as an unbiased com- parative control.