Effect of ozonated plant oil application on implant osseointegration in irradiated bone: an in vivo study

Abstract

Treatment of malignancies commonly involves radiation therapy. As a result of this therapy the vascular supply to irradiated structures is altered and results in decreased tissue perfusion. In addition to vascular changes, bony structures undergo alteration in osteoblastic and osteoclastic activity. These tissue alteration, enhance the risk of osteoradionecrosis. To avoid this occurrence, many patients who have undergone radiation therapy do not receive elective preprosthetic surgeries, including implant therapy. Ozone, an allotropic form of oxygen, possesses unique properties which are being defined and applied to biological systems as well as to clinical practice.purpose of the study: The purpose of this study was to evaluate the effect of ozonated plant oil on implant osseointegration in irradiated bone. material and methods: 32 titanium screw implants were placed into tibiae bone of 16 experimental New Zealand Albino rabbits assigned to groups I and II, both groups were further divided into 2 subgroups a and b, group Ia was considered control, In group II, the right and left tibiae were irradiated prior to implant insertion, both groups I b & II b received Topical ozonated plant oil. After 4 weeks animals were sacrificed and all specimens were examined under both light microscope and scanning electron microscope. results: Tibiae of the control group, revealed intimate contact of implant with bone in localized areas. Group I b showed intimate contact between the original well formed bone and implant. Architectural alterations were noted in the irradiated groups, where uncalcified newly formed tissue separated implant from bone. Application of Ozone in the irradiated group II b, resulted in the formation of newly formed osteoid tissue. conclusion: The use of implants in conjunction with topical ozonated plant oil in irradiated tissues may provide a mean of enhancing osseointegration.