Antimicrobial Profile of Selected Snake Venoms and Their Associated Enzymatic Activities
Shebl, R. I.
Mohamed, A. F.
Ali, Amal E.
Amin, M. A.
MetadataShow full item record
Aims: To investigate the antiviral and antibacterial profile of several crude snake venoms and to assess some of their enzymatic activities. Methodology: The antiviral activities of Naja haje, Bitis arietans, Naja nigricollis and Echis carinatus snake venoms were investigated against Herpes simplex virus type1, Rift valley fever virus and Vesicular stomatitis virus using the end point of cytopathic effect method. Antibacterial activities of Bitis arietans, Cerastes cerastes, Echis carinatus, Vipera lebetina, Naja naja, Pseudechis australis, Naja nigricollis and Naja haje venoms were examined against Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa using disc diffusion method. Microdilution method was used to determine the venom's minimum inhibitory concentration. L-amino acid oxidase and phospholipase A2 activities of crude venoms were evaluated using enzymatic assays. Results: Naja nigricollis, Bitis arietans and Echis carinatus snake venoms exhibited significant antiviral activities against all test viruses, except for N. haje treated cells. The mean depletion of viral infectivity titer of venom pretreated cells was higher than its depletion post viral infection for all three venoms showing antiviral activities. Naja nigricollis exhibited the highest antiviral activity against test viruses and recorded a mean depletion of viral infectivity titer in venom pretreated cells of 3.8 log (10) / ml , 3.2 log (10) / ml and 2.5 log (10) / ml for HSV-1, RVFV and VSV, respectively. Pseudechis australis, followed by Naja naja and Naja nigricollis venoms, showed the highest inhibitory activity against test bacteria with inhibition zones ranging from 11-17 mm, 8-14 mm and 8-13 mm, respectively. Minimum inhibitory concentrations of test venoms against different bacterial strains ranged from 156 μg / ml to 1.25 mg / ml. Maximum L- amino oxidase activity was detected in Naja naja, Cerastes cerastes and Pseudechis australis. The highest Phospholipase A2 activity was identified in Bitis arietans, Pseudechis australis, Naja naja and Naja nigricollis. Conclusion: It can be concluded that snake venoms or their bioactive derivatives can be promising therapeutic agents against some microbial infections. Further investigations will be carried out for purification and more characterization of the biologically active components in snake venoms.
- Research Paper 
Showing items related by title, author, creator and subject.
Towards discovery of novel scaffold with potent antiangiogenic activity; design, synthesis of pyridazine based compounds, impact of hinge interaction, and accessibility of their bioactive conformation on VEGFR-2 activities Jaballah, Maiy Y.; Serya, Rabah A. T.; Saad, Nasser; Khojah, Sohair M.; Ahmed, Marawan; Barakat, Khaled; Abouzid, Khaled A. M. (Taylor & Francis Group, 2019)Pyridazine scaffolds are considered privileged structures pertaining to its novelty, chemical stability, and synthetic feasibility. In our quest towards the development of novel scaffolds for effective vascular ...
Possible correlation between levansucrase production and probiotic activity of Bacillus sp. isolated from honey and honey bee Hamdy, Abdelhamid A.; Elattal, Nouran A.; Amin, Magdy A.; Awad, Ghada E. A.; Awad, Hassan M.; Ali, Amal E.; Mansour, Nahla M.; Esawy, Mona A. (Springer Science+Business Media Dordrecht, World J Microbiol Biotechnol (2017) 33:69, 2017-03-07)Five bacterial isolates from honey and bee gut were selected based on their high levansucrase activity and levan yield which were strongly positively correlated. All isolates showed good tolerance to temperature up to ...
Cerastes cerastes and Vipera lebetina Snake Venoms Apoptotic – Stimulating Activity to Human Breast Cancer Cells and Related Gene Modulation Ali, Amal E.; Shebl, R.I.; Mohamed, A.F.; Amin, M.A. (Journal of Cancer Science & Therapy, 2012-09-14)Apoptosis occurs normally during development and aging as a homeostatic mechanism to maintain cell populations. Dysregulation of apoptosis can disrupt the equilibrium between cell growth and cell death leading to the ...